南美白对虾肝胰腺蛋白提取方法优化及无水保活环境胁迫对细胞凋亡蛋白的影响

为提高Western Blotting结果的可靠性，以蛋白溶出率和凝胶电泳蛋白条带完整性为指标，比较提取液种类、研磨方式、酶抑制剂种类及其体积分数和提取时间对南美白对虾肝胰腺蛋白提取效果的影响。采用优化后的提取方法获得高质量蛋白样品，并采用Western Blotting法分析无水环境胁迫后南美白对虾肝胰腺组织中细胞凋亡信号通路相关蛋白的表达水平。结果表明：RIPA裂解液作为提取溶剂所得的蛋白溶出率高于水提和磷酸盐缓冲液，电动匀浆和液氮研磨所得蛋白条带更完整，4%蛋白酶磷酸酶混合抑制剂能有效抑制肝胰腺内源酶引起的蛋白降解；采用Western Blotting法分析无水保活期间南美白对虾肝胰腺蛋白，发现低温诱导休眠的同时会引起细胞轻微凋亡，且凋亡水平呈应激时间依赖性增加，环境胁迫解除后有所回调。     

自保护药芯焊丝明弧堆焊Fe-Cr-C-B-W合金的组织及性能

采用自保护药芯焊丝明弧堆焊技术制备五组不同钨含量的Fe-Cr-C-B-W合金. 借助金相显微镜、扫描电子显微镜、X射线衍射仪、洛氏硬度计和磨损试验机分析堆焊合金的组织及性能. 结果表明，合金的显微组织由马氏体、残余奥氏体、M₇(C,B)₃，M₃(C,B)，Fe₃W₃C和WC组成. 大部分钨元素被迁移到晶界生成了比WC稳定性更好的Fe₃W₃C缺碳复合相，堆焊层中没有典型的初生WC硬质相颗粒生成. 随着钨添加量的增多，共晶硬质相M₇(C,B)₃，M₃(C,B)和Fe₃W₃C随之增多，间距减小，呈连续网状均匀分布. 当钨的添加量为12%时，堆焊层的耐磨性达到最佳.     

Fabrication of improved flexural strength C/SiC composites via LA-CVI method using optimized spacing of mass transfer channels

The traditional chemical vapor infiltration (CVI) method still faces massive challenge in improving the densification owing to its unavoidable bottleneck effect. Herein, laser assisted-chemical vapor infiltration (LA-CVI) was introduced to fabricate C/SiC composites with mass transfer channels. As a result, the densities of the C/SiC composites were improved due to the dense band formed during the LA-CVI process. Also, with different spacing of mass transfer channels, C/SiC composites exhibited enhanced degree of densification varying from 2.10 to 2.23 g/cm³. When the spacing of channels was 3 mm, the maximum value of flexural strength reached 528 ± 12 MPa. Additionally, micro-CT and finite element analysis were empolyed to investigate dense band and density gradient in detail. The results show that C/SiC composites prepared via LA-CVI method with suitable spacing of channels had improved density and great flexural strength. The proposed method provides a novel route for the preparation of ceramic matrix composites with high density.     

Shape control and stability of multicore millimetre-sized capsules using a combined monoaxial dispersion electrospraying–ionotropic gelation technique

Multicore millimetre-sized fish oil-loaded alginate capsules were developed using a combined monoaxial dispersion electrospraying–ionotropic gelation technique and their stability was explored. By adjusting the preparation parameters, the capsule shapes could be irregular, spherical with a short-tail, spherical, fusiform, and fusiform with a long-tail. The continuous phase of the millimetre-sized capsules consisted of hydrophilic alginate calcium and water. Moreover, the water content increased from 17% to 69% with increased alginate concentration (2.5–30 mg mL⁻¹). The capsules prepared with alginate concentration of 10 mg mL⁻¹ or 20 mg mL⁻¹ show a similar loading ratio (about 5–8%) of fish oil during storage. Headspace solid-phase microextraction–gas chromatography mass spectroscopy (HS-SPME-GC-MS) results confirmed the capsules masked the fishy odour of fish oil. Moreover, fish oil slowly migrated from the inside to the outside of the capsules. This work presented a simple method to prepare multicore millimetre-sized capsules with controlled shapes and a basic understanding of the effect of encapsulation using alginate to mask the fish oil odour.     

CO2 sequestration in depleted methane hydrate deposits with excess water

The recent increase in atmospheric CO₂ concentration makes it necessary to investigate new ways to reduce CO₂ emissions. Simultaneously, natural gas hydrate mining technology is developing rapidly. The use of depleted methane hydrate (MH) deposits as potential sites for CO₂ storage is relatively safe and economical. This method can alleviate the shortage of hydrate displacement gas with CO₂. The purpose of this study was to investigate CO₂ hydrate formation characteristics during the seepage process—in reservoirs with excess water—and their effect on CO₂ storage. The experimental process can be divided into 5 parts: MH formation, water injection, MH dissociation, CO₂ hydrate formation, and CO₂ hydrate dissociation. Magnetic resonance imaging was employed to monitor the distribution of liquid water, and the effects of different parameters on the formation and dissociation of CO₂ hydrates were analyzed. It was found that a state of initial water saturation can effectively control hydrate saturation in artificial MH reservoirs for hydrate reservoirs with excess gas. In the process of CO₂ flow, initial water saturation was not the main controlling factor for CO₂ hydrate formation. Increasing the flow pressure and reducing the flow rate were beneficial for CO₂ hydrate formation. This study is of great significance for advancing the science of CO₂ geological storage in the form of deep‐sea hydrates.     

Some molecular aspects of hydrogen production by marine Chlorella pyrenoidosa under nitrogen deprivation condition in natural seawater

In this study, marine microalgae Chlorella pyrenoidosa produced 186 ml H₂/l under nitrogen deprivation in natural seawater, and adding 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) to medium reduced the total volume of hydrogen production by 85%. This suggested water was the main electron donor for hydrogenase. An active starch accumulation was observed during the first two days in nitrogen deprivation. But the starch content in cell decreased only by 7% at the end of the hydrogen evolution stage. This was shown the absence of a large contribution of starch to the hydrogen production by C. pyrenoidosa in nitrogen deprivation. Different from the hydrogen production by Chlamydomonas reinhardtii under sulfur deprivation condition, the concentration of acetate in the medium decreased not only at the stage of oxygen consumption but also during the stage of hydrogen evolution by C. pyrenoidosa. Thus, acetate is involved not only in the establishment of anaerobiosis but also plays an important role in the production of hydrogen by C. pyrenoidosa as an exogenous electron donor.     

Formable chromium nitride coatings for proton exchange membrane fuel cell stainless steel bipolar plates

Among the different coatings developed for proton exchange membrane fuel cell steel bipolar plate, nitride-based coatings present several advantages compared to gold or polymeric coating: high chemical stability, low interfacial contact resistance and reasonable cost. In this work, 50 nm thick chromium nitride coatings are deposited by reactive magnetron sputtering on 316L stainless steel foil. They are optimized to fulfill the Department of Energy targets in terms of interfacial contact resistance (ICR) and corrosion resistance, with values of 8.4 mΩ cm⁻² (at 100 N cm⁻²) and 0.10 μA cm⁻² (in 0.6 M H₂SO₄ solution at 0.48 V_vs. SCE potential) respectively. Moreover, they retain their excellent properties after high deformation (biaxial deformation of 20% in x-axis and 5% in y-axis), giving the possibility to achieve, in line, the stamping of a bipolar plate from a coated foil. The etching of the substrate, prior to the coating deposition, appears to be determinant to obtain low and stable corrosion current and ICR. The removing of interfacial oxyde leads to better coating adhesion and improves the corrosion resistance and electrical conductivity. The enhancement of the properties (low ICR and high corrosion resistance) is durable, with no signicant change of the ICR value up to 200 days after deposition.     

An improved partheno genetic algorithm for multi-objective economic dispatch in cascaded hydropower systems

The multi-objective economic dispatch (MOED) problem in cascaded hydropower systems is a complicated nonlinear optimization problem with a group of complex constraints. In this paper, an improved partheno genetic algorithm (IPGA) for resolving the MOED problem in hydropower energy systems based on the non-uniform mutation operator is proposed. In the new algorithm, the crossover operator is removed and only mutation operation is made, which makes it simpler than GA in the genetic operations and not generate invalid offspring during evolution. With the help of incorporating greedy selection idea into the non-uniform mutation operator, IPGA searches the solution space uniformly at the early stage and very locally at the later stage, which makes it avoid the random blind jumping and stay at the promising solution areas. Finally, the proposed algorithm is applied to a realistic hydropower energy system with two giant scale cascaded hydropower plants in China. Compared with other algorithms, the results obtained using IPGA verify its superiority in both efficiency and precision.     

Techno-economic analysis of a hybrid energy system for CCHP and hydrogen production based on solar energy

A typical problem in Northeast China is that a large amount of surplus electricity has arisen owing to the serious photovoltaic power curtailment phenomenon. To effectively utilize the excess photovoltaic power, a hybrid energy system is proposed that uses surplus electricity to produce hydrogen in this paper. It combines solar energy, hydrogen production system, and Combined Cooling Heating and Power (CCHP) system to realize cooling, heating, power, and hydrogen generation. The system supplies energy for three public buildings in Dalian City, Liaoning Province, China, and the system configuration with the lowest unit energy cost (0.0615$/kWh) was obtained via optimization. Two comparison strategies were used to evaluate the hybrid energy system in terms of unit energy cost, annual total cost, fossil energy consumption, and carbon dioxide emissions. Subsequently, the annual total energy supply, typical daily loads, and cost of the optimized system were analyzed. In conclusion, the system is feasible for small area public buildings, and provides a solution to solve the phenomenon of photovoltaic power curtailment.